I am making a standard curve of a platinum complex, the correlation coefficient that I get is around 0.94. I think one of the reasons is the fact that the drug is insoluble in water. If you have a better procedure of dissolving the drug, please share.
You can dissolve cisplatin in deionized water by vortexing it real well, but do not go higher than 2.5 mM, which is the limit. Before use, let the solution into a shaker for 10 min at RT.
Thank you very much Maria P De Miguel. My stock concentration was 5 mM, I stirred it for about 5 minutes, but it was not completely dissolved. So I would just swell it and pippette to make the next dilution. Someone suggested that I should leave the stock overnight and filter before using it. I am really sceptical about filtering, because I feel like it will be changing my concentrations.
You could dillute the cisplatin in DMSO first to about 10mM. it could be furthur dilluted with PBS before use.
Dear Simeng Chen,
I appreciate your response, however, I have read that DMSO disrupts the activity of platinum, so it is not advisable to use it in biological studies. Have you perhaps worked with it or do you have references that I could use?
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