How to calculate Ligand-active site distance in a ligand-protein complex from MD simulation?

I have run a MD simulation of a ligand-protein complex in GROMACS using CHARMM forcefield. Reviewer has asked me to calculate Ligand-activesite distance and binding free energy of ligand with...

27 February 2021 4,703 1 View

How to indicate the degree of litter decomposed by microbes?

Any technique could detect the degree of litter decomposed by microbes? Or any indexes could do this job? I want to split the contribution of microbes during decomposition, so any suggestions...

23 February 2021 1,362 5 View

How to Implement Elastoplasticity in a PCL model in COMSOL Multiphysics?

I am using COMSOL Multiphysics 5.5. I am trying to simulate the tensile test of PCL scaffolds. However, regardless of the strain, I have the elastic region all the time which is not true when it...

23 February 2021 4,050 1 View

Python - TRNSYS link not working with Type169?

I used Type169 for data exchange between Python and TRNSYS. Unfortunately, facing the following error. No proper documentation is available for this part. TRNSYS Message 105: A TYPE was called in...

14 February 2021 5,577 1 View

What is the role of ether solvent (such as THF) in Sonogashira coupling?

Can Amine (such as Et3N) be used as solvent as well as base for the Sonogashira Coupling? How to decide the amount of solvent to be used in any reaction? Does the amount of solvent affect the...

14 February 2021 4,018 3 View

Any natural extract (that is easily available) or any drug combination triple which i can use against gram negative?

I'm working on gram negative i want to use any natural extract (plant extract) or triple drug combination against its biofilm formation.

14 February 2021 1,403 3 View

Would bisulfide sequencing result be affected if the tissue is fixed by perfusion?

I am looking into DNA methylation of mice as an aging marker along with other assays and recently I added brain IF to the list of assays. For this, I need to fix the brain by transcardial...

09 February 2021 9,012 3 View

Why E1g mode (Molybdenum disulfide) is undetectable in Raman spectroscopy?

I synthesized 1T/2H hybrid phase MoS2 on a graphite foil surface by hydrothermal method, and then analyzed it by Raman spectroscopy. As a result, three new peaks correspond to the 1T phase were...

04 February 2021 6,580 2 View

Can I use 500ng of DNA for restriction digestion?

The recommended amount of DNA for restriction digestion is 1ug in a 50ul rx I've got around 500-600ng of DNA in a total volume of 35ul. Would I get satisfactory results with this amount of DNA for...

31 January 2021 729 6 View

Difficulties purifying Outer Membrane Vesicles using a Sucrose Density Gradient: Unable to detect any protein after this process?

I'm currently trying to purify Outer Membrane Vesicles (OMVs) following their extraction from the bacteria I'm working with. After the initial extraction the concentration of protein seems good...

27 January 2021 6,852 3 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

What is the optimal method to prepare a controlled porosity osmotic pump (CPOP) tablet for scanning electron microscopy?

In order to assess the shape and size of pores formed by dissolving the pore former in the semipermeable membrane structure, SEM images are needed. Cellulose acetate was used in the coating...

15 February 2021 6,135 3 View

Is carboxymethyl cellulose a better electrolyte host polymer than methyl cellulose and cellulose acetate?

I want to prepare a cellulose-based biopolymer solid electrolyte by solution casting technique. However, choosing the right cellulose derivative has been my major challenge. Among the many factors...

30 January 2021 5,355 3 View

What is the mechanism or interaction between Fe3O4 magnetic nanoparticles and biochar?

Lately there are many studies on biochar incorporated/loaded/coated with Fe3O4 magnetic nanoparticles. But I havent found any paper that describes the interaction or bonding between the two. How...

20 January 2021 3,162 4 View

After cellulose is regenerated in water, a cellulose hydrogel is formed. I want to know if there is crystallinity inside the the hydrogel?

After cellulose is dissolved and regenerated in water, a regenerated cellulose hydrogel is formed. I want to know if there is crystallinity inside the hydrogel, or it will not crystallize until it...

04 January 2021 7,466 4 View

How to calculate "POWER LAW" in rheology from a GRAPH of shear rate vs viscosity for cellulose nanofibrils suspensions?

How to calculate "POWER LAW" in rheology from a GRAPH of shear rate vs viscosity for cellulose nanofibrils suspensions?

02 January 2021 6,816 3 View

Crystallinity index measurement of Cellulose nanocrystals ??

How to find the crystallinity index of cellulose nanocrystals using XRD peaks. Can anyone suggest the most appropriate method of finding CI

28 December 2020 7,809 4 View

What is the best solvent and dose of Alloxan to induce diabetes in mice ?

Saline water or citrate buffer or carboxymethyl cellulose sodium salt

13 December 2020 4,110 1 View

What is the glucose content in Cellic CTec2?

Hi, I've determined the glucose content in Cellic CTec2 enzyme blend and it is 12-14 mg per 100 mg of enzyme blend. Could anyone share with me your results so I can compare it with mine?

08 December 2020 6,312 3 View

Reason for growth of fungus/mould in hydrothermal liquifaction filtered product of cellulose-based materials?

Performed hydrothermal liquefaction of cellulose powder, at about 200 degrees for 10 16 hours. After simple filtration, I stored filtered product at room temperature for 4-6 days, there is the...

03 December 2020 7,327 3 View