Does Covid-19 vaccine give to pregnant and breast feeding women?

I need to know the safety of Covid-19 vaccine in pregnant and breast feeding women

17 February 2021 504 9 View

An alternate method for z score which is better to be used in statistical analysis in cloud computing ?

I have used z score in one of my research paper. now i want to use some other good statistical methods. Need guidance in this regard. i am working in energy efficient algorithms in cloud computing...

21 January 2021 9,018 3 View

Can we use the general hotplate as a source of heating in the Soxhelt method or not ? what is optimal temperature when using n-hexane as a solvent?

there is a problem with the heating part in soxhelt apparatus, so my question can we use the standard hot plate as a source of heating?

05 December 2020 9,322 2 View

How can we calculate the concentration of nanoparticles in a solution ?

1- Absorption of 1 cc of a nm solution of 20 gold particles at 530 nm equals one. Calculate the extinction coefficient of these nanoparticles (consider the light path length of 1 cm). If we were...

30 November 2020 9,274 1 View

How can i collect many blood sample from the rat for pharmacokinetic study without killing the rat??

how can i collect many blood sample from the rat for pharmacokinetic study without killing the rat??

28 October 2020 2,675 8 View

How to calculate the quantity of enzyme needed to hydrolyze protein?

I have alcalase enzyme it's activity 10000U/g and I want to use it in hydrolysis whey protein isolate 90% protein. A suspension of 5% (w/v)WPI prepared so I am asking about the quantity of enzyme...

05 October 2020 9,825 3 View

Can oligo hexamer primer produce sequencing error?

Hello everyone I conducted sequencing prosedure for RNA genome segment (about 700nt) , for this reason I employed the random hexamer primer for cDNA synthesis . after sequencing (by sanger...

16 August 2020 3,994 3 View

What is the best way to teach applied sciences through E-learning?

I am an instructer at college of agriculture and I have difficulty to deliver the knowlege to my students in 3-learning especially in applied subjects and topics.

04 June 2020 8,757 14 View

Can a poor performance result be written for a paper?

I am currently working on a database and I have tried various methods of machine learning and deep learning, all leads to poor classification/prediction. However, this might show that the two...

27 May 2020 7,894 7 View

Is there any relationship between the density of surface charges of clay minerals with the activity of viruses in soil and Environment?

As we know some clay minerals and iron oxides have positive charges on Its surface, and viruses have negatively charges, is there any ability to use this feature to reduse or stop the activity of...

22 March 2020 5,007 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View