Hi everyone!
I want to use condylar cartilage (from TMJ) to do some experiments.
I read some papers and they only mentioned "harvest cartilage" simply.
I want to ask that is it easy to separate cartilage from subchondral bone?
I decalcified the whole head (cartilage-bone-tendon) and analyzed for cartilage or junction.
My previous answer was only for Histological or immuno studies. if you want other studies, that you can scratch with scalpel carefully. That is the only option I believe.
@Ka Tou
Hi
If you are planning to release cells from the cartilage you will need to enzymatically digest the mince
You can just shave the cartilage of the bone using a 22/15 /11 scalpel blade
If it's processing of just cartilage it should be pretty easy...as in routine processing
But if your cartilage material contains bone, it will require decalcification
And decalcification can mask the antigen binding sites and based on your antibody of interest you will be required to perform different antigen retrieval methods
Hope this helps
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