Ka Tou

6 Questions 3 Answers 0 Followers

Questions related from Ka Tou

How to dissect cartilage from condyle heads?

Hi everyone! I want to use condylar cartilage (from TMJ) to do some experiments. I read some papers and they only mentioned "harvest cartilage" simply. I want to ask that is it easy to separate...

31 May 2021 1,856 3 View

How to measure bone volume using data obtained by micro-CT?

Hey guys! I want to compare the bone volume of two samples, to know whether the bone was destroyed and bone erosions etc.. I scanned the bone using micro-CT. I found that in many papers they just...

04 March 2021 5,693 5 View

RNA concentration: Nanodrop vs Agilent 2100 Bioanalyzer?

Hi guys. I make RNA by in vitro transcription and do cell transfection afterwards. I analyze RNA using Nanodrop and Bioanalyzer. But I found the concentration of RNA measured by these two machines...

06 February 2021 869 3 View

How to do rat TMJ dissection and do micro-CT for condyle?

Hello, everyone! I want to ask how to remove TMJ from rat's head? Is there a protocol or instruction? And, if I want to do micro-CT for TMJ of rat, can I scan the whole head or after removal will...

23 March 2020 9,000 4 View

Should average value of control group's 2^-ddct equal 1?

Hello, everyone! So I performed the qPCR and when I analyze the results using delta delta ct method, in some control groups I can't get the result of 1, which might be 1.1 or 1.2 or even 3, can...

27 August 2019 7,256 1 View

Do we have to normalize results of ELISA by total protein assay?

Hi! I'm new to ELISA, and I'm confused about the methods for analyzing the results. I'm doing a very easy experiment. I'm going to check the amount of one secreted protein in the cell culture...

01 January 1970 4,217 5 View