I have been trying to develop a TLC system for an unknown compound (biosurfactant), but there are so many spots and trails in TLC and no solvent and their ratios have been proven appropriate for their characterization. I want to purify the compound by TLC cuttings so I need to develop a solvent system which is best suited for the separation of all the components present. Will you all please suggest some combinations of solvents which are universal for all compounds?

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