Killer cells were stimulated with anti CD3, CD28 to produce cytokines, brefeldin A was added but no expression of cytokines. any suggestion?
CD8+ T cells, NK, NKT cells were stimulated by anti CD3, CD28 for 1 hour, 4 hours, 24 hours to produce cytokines. Then, brefeldin A was added after stimulation times to keep cytokines inside...
01 October 2017
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