Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Is this method of modeling plastic behavior in Abaqus correct?

I've to model the plastic behavior (Yield Stress vs Plastic Strain) of a polyimide material (Elas_Mod=2500MPa) in Abaqus. The engineering stress vs strain plot is shown as the 3 red curves. I've...

03 February 2021 7,728 3 View

Which journals accept the short papers in the field of civil engineering or in the field of corrosion?

I want to publish my pilot study about corrosion in RC structures in any journal. Please help me to find good options.

25 January 2021 6,413 3 View

What are the insilico tools available to recognize transcription binding sites on a promoter ?

I have to control the expression of a gene ( in vitro ). For designing an optimal promoter assay, I would like to know more about Transcription factor binding region, enhancer and repressor...

11 January 2021 7,992 3 View

I have a histogram which I will be including in the attachment. Is this histogram of a bimodal image?

The image is basically a grayscale image having pixel intensities in the range 0-226. I have done image processing by converting this gray scale image into binary image using OTSU algorithm and...

10 January 2021 2,874 7 View

Which vegetable crops can tolerate salinity , Except Solanecious crops ?

Except ( Tomato-Brinjal-Capscum-Chilli) .

27 December 2020 8,362 12 View

How to convert RGB image of a stable diffusion flame captured from a mobile phone camera to thermal image?

I need the temperature profile (a quantitative dataset) of the flame under study. Is there any image processing tool a available which I can utilize for my study?

18 December 2020 2,707 2 View

Is there any noise present in raw LASER speckle pattern image taken from CCD camera ?

The Speckle pattern which Iam talking about is by considering the Fiber Specklegram sensors and when there is no external perturbation applied to the fiber. Here, fiber is MMF POF fiber.

17 December 2020 3,427 2 View

AAV2/6 and AAV2/2 purification by AVB troubleshooting?

Im trying to optimize my downstream process for AAV processing and I've been having quite a bit of trouble for some reason, I described my process below: I lysed cells in production media with...

30 November 2020 1,428 1 View

Working with nominal data in Excel / Stat plus, Chi square?

We are analyzing the response of >400 respondents to a survey, with values of -1 (no) 0 (neutral), and +1 (yes). The data were collected to assess opinions on feeding practices at zoos. An...

17 October 2020 953 2 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

What is the biochemistry of acidic solvents for phenolics extraction in strawberry?

I am using the Folin-Ciocalteu method for analysis of total phenolic content in ripe strawberries. I've read several protocol that suggest a methanol/H2O/formic acid (80:19:1) extraction solution....

22 February 2021 6,560 3 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

How to dissolve cross-linked Amberlite IRC120 H, hydrogen form resin?

Hello all, I have used Amberlite IRC120 H, hydrogen form acidic cation-exchange resin for the ion-exchange of waterglass [sodium silicate solution -- 40% Sodium silicate (mol ratio > 3.2)...

21 February 2021 5,614 3 View

Best protocol for immunogold using araldite resin ?

Hello, We perfom Tokuyasu method (ultrathin cryo sectionning) very often to make immunogold experiments. This time, we need to make a PLT technique with K4M or LR white resin. Our results were...

14 February 2021 4,959 1 View

How to improve the adhesion of epoxy coating on steel substrate? Which factor affects?

I am facing a problem regarding a de-adhesion problem with the coating. A brief description of my methodology is given below. I am using mild or Low Carbon steel as a substrate and I polish the...

14 February 2021 2,167 4 View

No title

The Folin-Ciocalteu method is an electron transfer based assay, and gives reducing capacity, which is usually expressed as phenolic content. Can anyone share any reference paper where total...

13 February 2021 3,782 3 View

Purified protein requires Calcium for activation, but the elution buffer I used seems to interact with Calcium, help?

09 February 2021 6,946 4 View

FLAG purification, elution doesn't have protein?

I am purifying a heterotetramer, with one subunit having a 1D4 tag and the other having a FLAG tag. I purified from Sf9 with HBS pH7.3 and 0.002%LMNG first with Anti-1D4 resin and got an adequate...

02 February 2021 3,860 1 View