THE RAPID, SIMULTANEOUS ANALYSIS OF 12 WATER-SOLUBLE VITAMIN COMPOUNDS.
Waters Corporation, Manchester, UK.
To utilize the power of Waters® ACQUITY® Ultra Performance Liquid Chromatography (UPLC®), along with fast mass spectrometric acquisition rates, to provide a rapid and selective method to simultaneously analyze 12 water-soluble vitamin compounds in one four-minute run.
What is your sample matrix? What is your expected concentration? What are your chromatographic conditions (column, mobile phases, extraction procedure...)?
Use a shorter exposure time or lower concentration for your degradation conditions. Your % Degradation should never go below 80% since your degradant molecules can undergo secondary degradation.