How to measure fluorescence intensity of Dapi stained nuclei?

I have tried various methods using imageJ to measure mean fluorescence intensity of dapi stained nuclei but none of them seem to be working. Ive attached an image i am trying to measure the...

15 February 2021 8,637 3 View

Dflux and urdfil subroutine input in abaqus?

I am using coupled transient thermal electric step to simulate thermal and electric flux load on cfrp layered composite. DFLUX been used for surface heat flux and URDFIL for element deletion but...

25 December 2020 2,551 1 View

How stiffness matrix modification after hinge formation is done for pushover analysis?

Can anyone please explain this? How do I account for plastic hinges in my model and how do I modify the stiffness after plastic hinge is formed? Need a small example. Thank You

15 November 2020 1,967 3 View

How to model hysteresis property of concrete in abaqus?

I want to model rc element subjected to cyclic loading, How do I enter hysteresis data in the material model? I want to use Mander's 1988 model to get data for concrete. I dont know how to enter...

06 September 2020 7,651 3 View

How to select appropriate value of dispersion coefficient for HecRas 1-D steady water quality model?

I am working on a water quality model for the Mula Mutha river. I have a doubt regarding the dispersion coefficient to be entered. whether to enter a fixed value or computed value? what are...

22 May 2020 848 1 View

Why the ZDOCK output file (.pdb) is not compatible for Chimera?

Why the final docked complex file output from ZDOCK is not compatible for Chimera?

24 April 2020 3,347 3 View

Got frequency of human recorded audio file in the range of 0-8000 Hz. Is it correct range?as per many sources human voice frequency is below 260 Hz?

I tried finding frequency by various tools such as audacity (got frequency 0-16000 Hz). Tried with Librosa(Python package) and got the frequency of human recorded audio files in the range of...

06 April 2020 7,607 6 View

Is there any software which take input as pdf(research papers) and generate the data from all the plots and graph inside it?(Similar to NLP)?

I am looking for software that will take input as a research paper (pdf format) and generate the data from plots and graph in it. as similar to NLP softwares.

04 February 2020 4,244 3 View

How to increase RNA yield and purity from PBMCs stored in RNA later at -80 C?

I have been using miRNA Isolation mini kit (qiagen 217004) for isolating RNA from PBMCs stored in RNA later at -80 C. The cell no. was 2 million cells. The RNA yields obtained are around 30-40...

15 October 2019 4,731 1 View

What is the criteria to select the range of normal stress in Direct shear test?

I did not not find the answer anywhere. Please help.

19 August 2019 9,950 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View