According to my previous pre-experiment method, I purchased 36 WISTAR rats for the wound recovery test(each rat with four wounds for different wound dressings). After the Wistar rats were purchased and reached a body weight of 300 grams, the rats were fasted for 12 hours to induce diabetes. STZ (Streptozotocin) was dissolved in normal saline containing sodium citrate as a buffer (10 mM, pH 4.5), and STZ (65 mg/kg) was administered to rats by intraperitoneal injection.
One week after induction, blood was collected to measure the fasting blood glucose value, and if it reached or exceeded 200 mg/dl, it was considered as having diabetes. After successful induction of hyperglycemic animals, follow-up experiments were performed.
Also, in my research, the mice blood sugar value can be measured with an Accu-Chek meter (Accu-Chek Guide, F. Hoffmann-La Roche Ltd., Basel, Switzerland).
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Thanks for your reply.We also follow almost similar process for induction of diabetes in our lab.But since a reviewer of the journal has special concern of how i determined the sample size and he wanted that we should have performed a priori sample size calculation to determine the appropriate sample size.Hence wanted information regarding it.
The STZ-induced WISTAR rats are just my pre-experiment. Still, as I mentioned, I use a total of 36 WISTAR rats as the total sample, which also allows me to use db/db mice with pre-regulated genes when publishing in journals, a total of 36 as model animals, maybe this link can help you.
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Thanks for your reply.We also follow almost similar process for induction of diabetes in our lab.But since a reviewer of the journal has special concern of how i determined the sample size and he wanted that we should have performed a priori sample size calculation to determine the appropriate sample size.Hence wanted information regarding it.