I used gravimetric analysis to determine microalgal lipid content after lipid extract in my lab. Now we need to determine both protein and carbohydrate content. We have a Velp TKN digestion-distillation which can be used to determine the estimated protein content of any sample. So, I am thinking if we extract lipid, then determine protein and subtract to find the carbohydrate content of microalgae sample, is this okay? Or
Is there any other direct method to determine lipid, protein, and carbohydrate?
Dear Md Khalekuzzaman
The most popular methods for the estimation of lipid, protein, and carbohydrates in microalgae are:
Lipid: Bligh and Dyer method 1959
Protein: LOWRY et al., 1951
Carbohydrate: Phenol–sulphuric acid method by Dubois et al., 1956
E.G. BLIGH, W.J. DYER, A rapid method of total lipid extraction and purification., Canadian Journal of Biochemistry and Physiology. 37 (1959) 911–917. https://doi.org/10.1139/o59-099.
LOWRY, O.H., ROSEBROUGH, N.J., FARR, A.L., RANDALL, R.J., 1951. Protein measurement with the Folin phenol reagent. The Journal of biological chemistry 193, 265–275. https://doi.org/10.1016/0922-338X(96)89160-4
Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F. Colorimetric Method for Determination of Sugars and Related Substances. Analytical Chemistry 1956; 28: 350–356.
I think this might help you
Thank you
Dear Sir,
You can use the Dubios method for estimating carbohydrates and the Kjeldahl method for estimating proteins. Thank you and good luck.
Best Regards
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