Dear Sasikanth Kommu,

Acid catalyzed transesterification is never attractive for lipids with extremely low free fatty acid (FFA) content. GC-FID analysis of transesterification product mixture (not the purified biodiesel) would give you information about the extent of conversion. However, I suggest you use product yield, rather than conversion. This paper may prove helpful Article Comparative effect of reaction time on biodiesel production ...

Regards!

Article Enhancement of Biodiesel Production from Marine Alga, Scened...

https://pdfs.semanticscholar.org/f7ee/a8ccebd600b936e913f07b208758de6eb016.pdf

https://surface.syr.edu/cgi/viewcontent.cgi?article=1002&context=bce_thesis

https://jchps.com/specialissues/Special%20issue4/jchps%2090%20s%20deepalakshmi%20263-265.pdf

Dear Onyeka Stanislaus Okwundu and Hamit Can,

Thank you very much for your help. I shall look into those papers and if I have any problem I shall contact you

Sasikanth

Perform an analysis of the lipid classes content before and after transestrification.

You can use Size Exclusion Chromatography for this purpose.

regards,

G

Dear Grzegorz Boczkaj

Thank you very much for your suggestion

Sasikanth

In the literature there are several procedures regarding the yield / conversion of the oil after the transesterification process. One of the techniques used is the percentage of methyl esters found after injection into the gas chromatograph (GC). For this, it is important that you do the methodology using internal standard, using methyl heptadecanoate (C17). Since you only want to know the conversion of the oil into biodiesel, there is no need for more standards for the identification of the methyl esters produced.

Best Regards

Go, for NMR test. I shall help you after that. It is easy.