What is the difference between dose and concentration? What is the dose and concentration of a killed vaccine and a live vaccine?
dose of vaccine is the volume need to induce an efficient immunity in target species following the inoculation.But concentration basically means the mass of API in desired range, micro(nano)gram/ml.
I agree with Ali Moradi about the dose that is the volume needed for one immunization, but the concentration in live and inactivated vaccine is usually concentration of bacteria or virus.
Let’s suppose you have an inactivated bacterial vaccine, which has concentration of bacteria 10*7 CFU per ml. Or a live or inactivated viral vaccine, the concentrations of the virus is in PFU, TCID50 or PRNT50. In case of sub unit, peptide, protein or polysaccharide vaccines, concentration is in µg, ng or pg.
The dose can be optimized by safety, concentrations, uptake capacity and immune response.
Hope it has answered your doubts.
Wish you all the best
What are the basic calculations of vaccine doses for immunization? It should be possible to guess the amount needed before the production is done to determine the cost.
The dose of the vaccine is decided on the basis of fundamental immunological knowledge and preclinical studies.
While doing animal studies, we optimize different doses to check the maximum immune response and challenge studies. Once the standardization and clinical trial are completed, it can be used for the final product. Dose optimization Process is similar to both live and killed vaccines.
Vaccine development takes a long time before reaching the marketplace, after marking all the parameters only any vaccine is allowed to use in humans. Even the vaccines which are already in the market needs to clear many Quality control parameters before releasing into the marketplace.
According to conventional methods, one dose of killed vaccine should be able to induce produce of antibody in target species which its unit usually expressed as 1 IU/ml of serum. This mostly determined by challenge in vitro tests or alternatively by ELISA which takes more less time.
- Badges
- Science topic
- Similar topics
- Clinical Pharmacology
- Dosing