Determine the dry weight of the biomass by oven drying, and ash the dry biomass in the muffle furnace. Based on the ash weight obtained, ash-free biomass can be calculated. Usually for a commercial production system, calculation of dry biomass may be helpful for marine cultures in particular. And in case of biofuels, determining the ash content will help us to know the actual carbon availability in the biomass and its fuel conversion efficiency. Sometimes the composition of ash may also affect the downstream processing of biomass for biofuels.