Is it strictly recommended to run samples in duplicate during SNP genotyping analysis?

02 February 2021 8,877 3 View

Genus name priority from the same publication (Zoology)?

I came across a recent taxonomic work, where two genera were synonymized. Both genera were published in a paper in 1922 by the original author who revised the family at that time. The original...

19 January 2021 1,920 4 View

What are state-of-the-art methods/techniques/algorithms for the root-cause analysis of the anomalies?

I have been looking for research articles that target the question "why did an anomaly occur?", however, most of the papers that I found are related to "if an anomaly occurred?". I searched Google...

12 January 2021 7,951 3 View

How to present and discuss results, when methodology is not data-based?

Dear Everyone! I'd ask you opinion about a problem, which is returning in my "softer" research field. Time to time I work on research projects, which do not have available data and/or the data...

29 December 2020 7,637 6 View

How would you describe the most relevant research questions and directions in urban air pollution, if I'm specially interested in transportation?

I am relatively new to this field, my research interests are smart cities, shared mobility, transport service integration. We have data about CO, NO2, O3, PM10 from several cities. I would like to...

11 November 2020 9,099 4 View

Trizol samples wont freeze. Can it cause problems?

Hi, everyone. I recently lysated cell culture with Trizol reagent, and I put my samples in -20C. But, some of my samples wont freeze, and I can see two separete phase appearing in them. I'm awaer,...

06 November 2020 10,002 1 View

How to amplify lignin degrading gene?

Hello colleagues, I would like to amply the lignin-degrading gene (i.e., LigX, LigY, LigZ, and LigW) from different types of soil. I have tried to find literature, but unfortunately, there are...

20 October 2020 9,654 3 View

How to remove abnormal behavior at support of FE model of RCC Beam?

Hi, I am modelling a half model of RCC beam in ANSYS Mechanical APDL. The model I have created is exhibiting abnormal behavior at the support with abnormal cracks. I would be grateful if you...

12 October 2020 8,596 3 View

How to calibrate the parameters of coupled damage-plasticity microplane model of concrete for FE modelling of RCC beam in ANSYS Mechanical APDL?

Hi, I am trying to model an RCC beam in ANSYS Mechanical APDL. I have the value of Compressive strength, and modulus of elasticity of the concrete of the experiment I am trying to validate ? Can...

28 August 2020 4,623 2 View

Culturing CHO-freestyle cells without shaking?

Hi everyone! Recently I started working with CHO-Freestyle suspension cells, but our laboratory does not have an orbital shaker. I tought it would be okay without it, so I just used a normal T25...

20 July 2020 4,597 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View