How to determine LOQ in real time PCR standard curve?

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Bhairavi Vajaria

Article Methods to determine limit of detection and limit of quantif...

Alomgir Hossain

The calculation method is again based on the standard deviation of the response (SD) and the slope of the calibration curve (S) according to the formula: LOQ = 10(Sy/S). Again, the standard deviation of the response can be determined based on the standard deviation of y-intercepts of regression lines.

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