How to determine dCas9 (Nickase) cut efficiency?

23 July 2019 1 3K Report

I was wondering if there is any quick tool to quantify the cut efficiency of a nickase strategy. I was thinking about TIDE, querying it using either gRNA. Would you think this is a meaningful approach?

any other suggestion?

Thanks

Michael Kosicki

TIDE might work, if you use two gRNA in close proximity, if it aligns the WT/mutated traces correctly. Make sure to change the default settings on the size of deletions. If it's very efficient, you will just see the result in the Sanger traces.

If you want to test individual gRNAs, there's a number of options, let me know.

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