I have few queries regarding Assessment of invitro antiinflammatory activity Inhibition of albumin denaturation???????????
1. What is the total volume of reaction mixture? And how much extract we have to take? And how much 1% aqueous solution of bovine albumin fraction is to be added?
2. How much HCl we have to use and what is the final pH expected?
3. After all I am getting absorbance of control above 1 . So can i dilute all my samples and control to get the absorbance below 1? Will there be any change in inhibiton % ?