How to detect the compounds present in an aqueous extract of a plant material on tlc? And please suggest me ratio to extract?

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The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

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Faiq H S Hussain

It can be detected by Iodine vapor. Put some I2 crystals into small glass container and close the bottle gently till it is saturated by I2 vapor, then put the TLC plate into the bottle and reclose. The spots will appear as a dark colors.

Imran Ali

thank you

Chen Jingwen

Use Iodine or potassium permanganate or other compounds.