As we know the use of substrates such as Nile red, EtBr and EPI's such as CCCP helps us to detect RND efflux pumps in E.coli. How can we detect TetA/B/C (Tetracycline resistant) or FloR (Chloramphenicol resistant) efflux pumps in bacteria? Are there any proposed protocols in the literature?
The presence of efflux pump genes in Escherichia coli could be detected by PCR assay using appropriate primers. One can also use experimental systems whereby the pump protein is over-expressed and substrates are identified by increased resistance to a panel of compounds Article Efflux pumps of Gram-negative bacteria: what they do, how th...
https://aac.asm.org/content/45/4/1126
You should use the specific primers in PCR analysis which detect tetracycline and florfenicol resistance. When you get a positive result, you could mention the resistance of tetracycline and florfenicol if the genes encoding the efflux pump. If you want to see gene expression rate for specific efflux pump working rate, you should quantity the genome expression rate in qPCR.
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