Heather A. Leslie et al. Preprint Journal Pre-proofs Full length article Discovery and quantif...
tested a method for measuring the concentration of plastic in the blood.Scientists took blood samples from 22 healthy volunteers without any specific eating habits. Blood was taken from a vein into a glass tube, which was sealed with an isobutylene-isoprene rubber stopper (this material was not one of the target plastics in the experiment). Moreover, the blood was drawn directly into a test tube to minimize its contact with the air in the medical office. All medical instruments, from needles to test tubes, were previously sterilized, and their material was taken for analysis in order to then cut off its signal when processing analysis data.
The blood was then frozen and sent to a laboratory for analysis. There it was first thawed, then one milliliter was taken, which was heated to 60 degrees Celsius to denature the proteins, and proteinase K was added (this is a standard procedure in molecular biology for purifying drugs from protein impurities). The sample was then passed through a glass filter with a mesh size of 700 nanometers.
Everything that remained on the filter went into the chromatograph. The samples were heated to 300 degrees Celsius, their composition and concentration were determined by gas chromatography and mass spectrometry. In parallel, exactly the same measurements of the composition of 16 “seasoned” samples were carried out - 10 micrograms of a mixture of target plastic particles were added to eight, and 75 micrograms to the rest. Eight more measurements were carried out with ordinary water as a control.
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