Can anyone show me how to use NH4OH consumption volume of Pichia pastoris to indicate methanol toxic level?
I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...
10 August 2024 4,005 2 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
Hi all, Do you know how to plot this figure? I found many of them in Nature journals but have not figured out how to make them. Which software/tool do you suggest? Thank you very much.
15 July 2024 8,384 2 View
Hello everyone, I am new to LabVIEW, right now we need to control the movement of an ethernet-based stage drive. We got the sample code from a collaborator but their instrument is USB-based, so we...
11 July 2024 5,385 2 View
I utilized a Bayesian state space model to analyze the data. The 95% confidence interval, which ranges from 0.0 to 1.2, prompts the question: Is the regression coefficient significantly different...
01 July 2024 9,575 2 View
How to calculate Module Young from compressive strength and strain curve
29 June 2024 817 3 View
The software used for genebank to store and manage information, it could be free or licence (friendly to use)
24 June 2024 285 0 View
I prepare Mg doped Hydroxyapatite by wet preipitation but (Ca + Mg)/P ratio is higher than 1,82. CaO peak cannot be detected in XRD spectrum
23 June 2024 8,970 19 View
I've been fabricating traction force microscopy devices in glass bottom dishes, using a method that first spins a 100 micron PDMS layer, then a ~1 micron PDMS + fluorescent beads mixture layer....
09 June 2024 652 2 View
I need some technology applying in cvt gearbox due to improve the effective of this type
11 April 2024 9,582 0 View
Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you
24 July 2024 3,937 0 View
im studing gaussian 16 with reading paper about I-131 Metaiodobenzylguanidine in the paper "In a similar vein, nuclear magnetic resonance shielding values were investigated using the widely...
16 July 2024 6,040 4 View
I am searching for HPLC method to estimate Mirtazapine but i want to avoid HPLC-buffers as a mobile phase component. So, I am looking for alternative HPLC-solvent (acetonitrile, methanol etc.)...
14 July 2024 1,493 3 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
I need to do a Schiff base reaction starting from a ketone and 1,10-phenanthroline-5-amino. I've seen that both of these starting materials are not very soluble in methanol and ethanol, which is...
23 June 2024 5,925 3 View
I have been using Zeocin marker for target protein expression to verify integration in Pichia system. However, I would like to remove Zeocin marker since it's antibiotic related and not ideal for...
15 June 2024 3,851 2 View
A methanol production process combines tri-reforming of methane (TRM) with water electrolysis to utilize CO₂. The TRM reactor uses a Ni/Al₂O₃ catalyst, and the methanol synthesis reactor uses a...
10 June 2024 2,105 1 View
I work on the synthesis of Schiff base. I am trying to synthesize a schiff base from salicylaldehyde & para amino benzoic acid. But I face a challenge to purification of this product. I can't...
02 June 2024 1,576 8 View
I am trying to integrate a 11 kb DNA, linearized with AvrII, at GAP locus in Pichia pastoris genome. I am using electroporation for the same. After transformation, I am observing colonies after...
27 May 2024 4,777 2 View
I have 2 2 2-trifluoroethyl methacrylate polymer solution and I want to separate polymer from solvent , I used methanol but my polymer solution in methanol seemed cloudy . How can I precipitate...
10 May 2024 6,823 4 View