How to calculate age-standardized prevalence ratio with 95%CI?

I need to calculate the prevalence ratios to show the trend of prevalence of a drug group used by pregnant women over the years (2001-2018). I would like to use the year 2001 with age distribution...

05 January 2021 7,986 5 View

Does CD31 indicate cell viability?

Hi, I am wondering if the immunofluorescent staining of CD31 antibodies can both mark endothelial cells and indicating their viability. In other words, if I perform immunofluorescent staining for...

23 December 2020 5,815 4 View

How to increase titer of Herpesvirus, Adenovirus?

I'm working with Herpesvirus and Adenovirus. For Herpesvirus, I use Vero cell line to activate and check virus titer. For Adenovirus, I use Hela cell line. Recently, I could activate them, but...

16 December 2020 4,073 5 View

How big can a enhancer deletion be ? how can we obtain homologous deletion clone ?

Hi all, I have tried to use Crispr Cas9 ( plasmid px333_cas9_mCherry) to delete some enhancers. I designed sgRNA using Chopchop website to target both sides of an enhancer to induce homologous...

13 December 2020 942 3 View

How to calculate the fractional vegetation cover (FVC) from Landsat imagery?

Hi folks, I wonder if anyone know how to calculate the fractional vegetation cover (FVC) from Landsat imagery? As far as I have read about that NDVI, NDVIs, and NDVIv are three required input...

07 December 2020 4,054 2 View

Is it possible to identify/classify rubber using satellite imagery with machine learning?

As of now, I am trying to explore the potential of using high/very high-resolution satellite imagery with machine learning (ML) to identify or map forest cover (e.g. rubber). As a newbie in this...

17 November 2020 7,187 3 View

Cloudy brain samples in sucrose solution?

I have some samples I left in sucrose for a couple of weeks after perfusion and was wondering if these samples are still salvageable? I perfused the brain, then they were fixed in 4% pfa for 24...

15 November 2020 1,728 2 View

What is Snapback Primer in realtime PCR?

Dear all, I find a paper using Snapback primer. I do not understand the function of snapback tail? What is snapback primer? When do we use snapback primer? and why we use that? Thank you.

01 November 2020 8,869 2 View

CDNA PCR problems: why did the sample without reverse transcriptase enzyme (band 5 in both two genes) have strong band with gen A?

Dear all, These are data from gel base of cDNA sample for PCR with protocol attached on the figure. Band 5 is no reverse transcriptase enzyme during doing cDNA library used random primer. With gen...

04 October 2020 8,774 4 View

Why were cDNA PCR got multiple bands when added DMSO?

Dear all, All samples are cDNA, using reverse transcriptase enzyme to make cDNA from RNA. One group is doing PCR without DMSO, and other group is doing PCR with DMSO. One is without DMSO, the...

04 October 2020 1,320 3 View

What is the indication of the absence of peaks in Methanol electrooxidation CV curves ?

I have several question regarding the MOR; hope anyone help me better understand. 1- in CV using (KOH+MEOH) what is the indication of absence of the redox peaks? shouldn't they exist as a sign...

28 February 2021 4,851 8 View

(western blot) can methanol damage my antibody signal?

I accidentally rinsed with methanol after adding my primary antibody to my membrane. I noticed after ~10 seconds and immediately replaced it with PBST (3x15 min washes). Will my antibody signal be...

12 February 2021 5,824 3 View

Why do my polyphenol measurements fail?

In order to measure the polyphenols in broccoli, we prepared a reagent for measurement using the following procedure. 1. Extract the crushed plant with 70% methanol. 2. Mix 1 ml of the extract...

07 February 2021 845 4 View

How to calculate Total Phenolic Content and express it in mg GAE/kg?

26 January 2021 3,847 7 View

GC-Headspace (HS-GC) chromatography for Forensic toxicological and Food expertises?

Dear Colleagues! How correct and appropriate is it to conduct forensic toxicological and food examination of volatile substances (ethanol, methanol, propanol, ethyl glycol, etc.) in blood and...

24 January 2021 5,645 2 View

Gibbs and fuel cell?

20 January 2021 6,146 2 View

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16 January 2021 8,334 5 View

Methanol usage in determination of vitamin B2 in foods using HPLC?

After acid hydrolysis and incubation in the dark using Taka-diastase enzyme, 30% sulphuric acid was added to stop the enzymatic reaction. The solution was diluted to the mark and some of the...

16 January 2021 3,740 4 View

How can I analyze using NMR spectroscopy; my compound is only soluble in hot methanol?

Halo every one I have isolated a compound from the methanolic plant extract. The compound is UV positive at both 254 and 365 nm. It seems to be insoluble in most solvents like CDCl3, CD2Cl2,...

13 January 2021 2,267 3 View

What is the solubility of Magnesium trifluoromethanesulfonate in methanol?

I am trying to dissolve Magnesium triflate in methanol. I took 2.6 g of it in about 20 mL methanol. After thorough stirring, I can see suspended particles! Can anybody throw some light on its...

12 January 2021 6,481 2 View