Quang Lộc Trần

4 Questions 7 Answers 0 Followers

Questions related from Quang Lộc Trần

Why my gels got different distributes when I change acrylamide?

Hi researcher and fellows, the protein bands separated differently as compared in two gels (12.5%). The sample for both is the same but it looks like weird in the second gel. That gel did after I...

29 March 2020 7,909 4 View

How to enhance yeast growth rate (Pichia pastoris) and adjust proper methanol feeding rate without sensor?

Hi fellows and researchers, I am doing Pichia pastoris fermentation, GS115 strain containing pPIC5a, 50-L scale. I follow the Invitrogen protocol and try hard to get the recommended biomass before...

08 December 2019 8,878 3 View

Poor protein signal of gel by silver stain SDS-PAGE

Hi fellow colleagues and scientists, I have some troubles staining silver gel after running SDS-PAGE. 1. I can't get full of protein signal on gel that makes a blank in the end of the gel....

01 January 1970 1,039 6 View

How to detect methanol accumulation in Pichia pastoris fermentation broth without sensor?

Can anyone show me how to use NH4OH consumption volume of Pichia pastoris to indicate methanol toxic level?

01 January 1970 759 0 View