I an having problem in developing the blot. As i can see bands with Ponceau, but after primary and secondary Ab incubation the blot shows no result. the primary Ab is freshly prepared. These Ab are working fine before.
As i have changed every chemical, but there is no result. so, i am thinking that the memb may get dried (How, i don't know).
Does the dried blot will show band with Ponceau stain or not. Because i have seen band with Poncaeu but can't develop the blot.
Rafik Karaman
Dear Shradha Mawatwal,
I refer you to the following link which contains good answers to a similar question to yours:
https://www.researchgate.net/post/Problem_in_Ponceau_staining_after_protein_Transfer
Hoping this will be helpful,
Rafik
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