How to design read out circuit for electrochemical sensor?

More Tasneem Harahsheh's questions See All

How to minimize the standard deviation of protein-ligand complex using mmpbsa (CaFe 2.0 vmd plugin) using namd?

Hello, I wanted to know how can I minimize the standard deviation (SD) of my simulated protein-ligand complex of 50ns using MM/PBSA method of Cafe 2.0 plugin of VMD plugin using NAMD. I have tried...

10 December 2020 8,132 1 View

How to clean PTFE vessel from carbon deposits after it has been used for generating carbon from glucose?

A PTFE vessel was used as a reaction vessel to carbon coat magnetite NPs using glucose at 180C for 12 hrs. The interior of the vessel was blackened by the carbon deposits. It was soaked for long...

15 October 2019 2,326 3 View

How to clean PTFE vessel from carbon deposits after it has been used for generating carbon from glucose?

A PTFE vessel was used as a reaction vessel to carbon coat magnetite NPs using glucose at 180C for 12 hrs. The interior of the vessel was blackened by the carbon deposits. It was soaked for long...

09 October 2019 5,570 3 View

What is the configuration file for blind docking using PyRx for multiple ligands?

I am trying to run blind docking with multiple ligands and one macromolecule using PyRx but somehow i am getting poor binding affinities. What is the configuration file for blind docking in PyRx...

01 April 2019 2,132 3 View

How to deposit gold nanoparticle onto (commercial) disposable screen printed carbon?

i have carbon screen printed electrode and gold nano particles suspension in citrate buffer, how to deposit the particles on the electrode in a way to get uniform distribution?

28 February 2019 9,323 3 View

If i have protein amino acid sequence how to get the 3D structure?

i have amino acid sequence of protein and I am looking for a free tool can predict the 3d structure, also i have the aptamer of this protein and i want to know how they bind with each other ?

16 February 2019 5,364 5 View

What is the best method to synthesize gold nanoparticles from gold sheet?

Gold nano particles have wide application range. One of the main problems of gold nano particles synthesis is producing well dispersed non agglomerated particles? I greatly appreciate your suggestions

31 December 2018 2,220 6 View

Is it possible to desulphurize shale oil while in gaseous phase using non hydrogen methods?

Shale oil has plenty of sulphur in the form of thiophenes, thiols etc. Its desulphurization is a critical issue for its use. I am really interested in insitue desulhurization during pyrolysis

10 November 2018 7,067 0 View

What is the basic different between IDA and SPE?

Currently Iam working on amperometric biosensor using SPCE (screen printed carbon electrode) but I still confuse to figure out what is the different between this electrode with Interdigitated...

04 November 2018 7,738 2 View

HMM for maternity dataset using R?

I have attached the simplified form of my data. The variable "Gender" will be the hidden state in our model and "Pregnancy Outcome" will be the observation. What is confusing me is that I don't...

18 September 2018 3,527 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Gas sensor response and recovery time independant of flow rate and chamber volume?

I am trying to obtain the response and recovery times for my gas sensor device. It appears the the response/recovery times are primarily dependant on the flow rate of the gas I inject into the...

26 February 2021 1,511 3 View

How to use measure capacitance change in COMSOL time dependent study ?

I want to measure change of capacitance due to relative displacement between two parallel plates in COMSOL. Distance between two plates changes with time. So I'm using a time dependent study but...

26 February 2021 8,567 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Mitchai Chongcheawchamnan

Seems like u are making decision on choosing either DC or AC data.

Umer Farooq

Resistance is a value which measure how much the component “resist” the passage of electrical current while capacitance is the capacity to store energy in a capacitor. In your vase do EIS, CV etc using electrochemical workstation to check the activity of your electrode