Dear Tasneem,

You can go through our paper where we have developed an electrochemical sensor for breast cancer biomarker detection. You will find all the guidelines for designing an electrochemical sensor in the manuscript. Let me know if you have any questions. I would be happy to help.

Article Integrated Dual-Modality Microfluidic Sensor for Biomarker D...

Hi !

Get a look here :

http://journals.sagepub.com/doi/pdf/10.1016/j.jala.2010.01.007

Hi Tasneem,

A few topics to consider to develop a electrochemical biosensor:

1) Probe-target (DNA-DNA, enzyme-substrate, etc.)

2) Type of electrode

3) Immobilization of probe (surface modification, probe modification)

4) Electrochemical technique

5) Validation of probe immobilization and target detection

6) Calibration curve, sensibility, limit detection, seletivity

The order of the topics is relative (i don't know what you have in mind or in your lab), but in general all this must be evaluated to produce a sensor with good performance.

I hope I was helpfull and good luck!

THANKS IN ADVANCE

Dr.Leandro what do you mean by electrochemical technique?

Hi!

I mean what methodology you'll use to develop your biosensor and the methodology for biological recognition detection (probe-target).

For example: cyclic voltammetry, differential pulse voltammetry, SPR, impedance spectroscopy, etc.

Leandro Toshio Kochi hello again !

in cyclic voltammetry i will use a Potentiostat to provide a constant voltage stemulat the sensor during the masurment, my quastion how to chose this voltage value? have to set the Potentiostat with a DC value , but i dont know from where ican get it

if you have any idea pleas let me know, thanks in advance

I don't know if I understood your question, but in cyclic voltammetry the potential range is fixed (for example -0.6 to +0.8) and in each potential point the current values changes, I don't know if it's possible to provide a constant voltage in this method.