I use CFD-post to define the Nusselt number. I defined it based on the dimensionless temperature gradient, but I found that the dimensionless temperature gradient in cfd-post is 0 everywhere. what shoud i do?
I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?
23 July 2024 1,353 4 View
Some time ago, I wanted to perform immunofluorescence on the S protein of mouse CD4 T cells using a Leica super-resolution microscope. I fixed with 4% paraformaldehyde, then added it to the well...
20 July 2024 3,797 2 View
Hi all, I am trying to use mixed effect model to analyze my data, which including a baseline measurement for my exposure (A), and repeated measurements for the outcome (B). I do have some...
17 July 2024 8,682 3 View
Dear colleagues, As well known, we can deliver messages in different languages and characters, including Chinese, English, Latin, binary and decimal codes (if with a converter) and many other...
08 July 2024 5,422 13 View
Hello, I am experimenting to grow cells onto permeable membrane support (inserts) in a 12-well plate. The cells are stained with DAPI. Just wondering how to image the cells attached to the inserts...
20 May 2024 1,061 0 View
Use of thioglycolic acid as a stabiliser during the synthesis of nanoparticles
05 May 2024 9,109 1 View
It can be found in most literatures and reviews that METTL3 exerts its methylase activity in the nucleus, interacting with adaptors such as METTL14 and WTAP, but is it possible for METTL3 to play...
09 April 2024 9,801 0 View
Now I need to carry out the task of frequency calculation on a large number of models, because only one CPU per job for frequency calculation, so in order to improve efficiency I have to submit...
09 April 2024 652 2 View
This is the first time I did an H&E staining, and I found that there are some white (blank) spaces between the cells. I do not think this is the normal morphology of this tissue. Here are...
08 April 2024 8,491 2 View
I am an international and a first-year undergraduate Medical Science student at UNSW. How can I get involved in research as soon as possible, and if I can't, what should I do to ensure that I am...
29 March 2024 4,934 1 View
In cases where the rotational of the magnetic field H is zero, we can define this field as the gradient of a scalar function defined as the magnetic scalar potential (similar to the electric...
21 July 2024 9,633 4 View
Where is the stream gradient usually greatest and relationship between the slope of the stream channel and the velocity of the stream?
13 July 2024 5,825 0 View
How do gradient and discharge change in a downstream direction and why are glacial valleys shaped differently from river valleys?
13 July 2024 9,228 3 View
What happens to the stream's discharge if the gradient of a stream increases and relationship between the gradient of a stream and the rate of water flow?
13 July 2024 5,085 2 View
How does that stream's gradient change downstream as it enter the alluvial fan and difference between an alluvial plain and a delta plain?
12 July 2024 7,337 0 View
How does stream gradient steepness change moving downstream and gradient of a stream change along its longitudinal profile?
12 July 2024 4,113 0 View
I am following the NEB protocol to perform SDM with Q5 and NEB HiFi Assembly. I am making a two base pair change. First picture is my Primers designed with NEBaseChanger. Second Picture is my...
24 June 2024 6,079 4 View
Hi, I have recently ran a duplex ddPCR EvaGreen assay using different primer concentration for target (150nM) and reference (50nM) primer. The assay was ran on a XY sample for SRY copy number...
22 June 2024 9,200 3 View
Hi everyone! I am using a Q5 high fidelity DNA polymerase to amplify a 6.7 kb plasmid. The PCR with Q5 went well with longer primers (~55 bases) at 70-72 degree C. However, I keep failing in the...
17 June 2024 1,734 4 View
Hi y'all, does anyone know if animal cells increase in size as they age or do they simply multiply? I usually do a Percoll gradient centrifugation to isolate endothelial cells but with pups it...
07 June 2024 4,065 1 View