Hello, I need some help with analyzing the ABR (auditory brainstem responses data) of mice. However, I am confused that on which basis should I take wave as wave 1?
there are two scenarios,
1- Is there any specific range for wave 1 amplitude (in microvolts) to consider it as wave 1?
2- Should I take the very prominent wave in the ABR waveform as my wave 1 every single time?
Please provide me with the guidelines or any standard method to deduce wave 1 in the ABR waveforms.
I will highly appreciate your guidelines.
Thank you very much.
Hello, in my opinion, Wave I generally comes around 1.5 ms in humans but because you are doing it on a mouse, it might come even more early, so there is not a particular amplitude value to deduce Wave I but what you can do is you can trace wave V, wave III and wave I at high intensity, so you will see three peaks with a gap of 1-2 ms in between them then keep reducing the intensity and you will be able to trace that those three peaks (if they are the real Wave response) they will start shifting in latency with decreased intensity so you can trace them moving slightly ahead with decreasing intensity and this will help you to be sure that this is a wave I, wave III and wave V of ABR.
hope it helps!
Thank you very much for your answer, so, I can take wave 1 with 0.8 or 0.78 ms latency. As In my case, the wave 1 comes earlier and if I go for the most prominent wave as my wave 1 then the latency increases and this changes the difference between control and diseased group.
Yes if you can see the prominent peak around 0.8 or 0.78 ms then you can trace it by changing the intensity if you give a louder intensity the latency will decrease and if you lower the intensity the latency will increase, so if you can trace that prominent peak slightly shifting with varying intensity then you can be sure it is wave 1.
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