How many ratio of simplicia powder : water I should add to make the infusion concentration 100%?

I am doing research to test antibacterial activity using plant leaves that I have mashed. How many ratio I should add between the simplicia and water to get 100% concentration in infusion technique?

06 December 2020 1,010 6 View

How much (sea)salt is needed to stop the turnover of the cold fresh water melting from the "ice"-poles?

The cold fresh water from the poles is going to block the warm water (Gulf) stream (heat transport). There is a theory that salt in water can conduct electricity. So it could start some electric...

19 September 2020 7,712 2 View

Does Covid-19 effect certain parts of the brain?

Dear Colleagues, I like to know if Covid-19 effects the brain in general, depending on person's constitution or has Covid-19 a 'taste' for certain brain areas? Thank you for answering Kind...

27 July 2020 7,159 20 View

Human mesenchymal stem cell differentiation issue?

hello, i got a problem in the adipogenesis. The human MSCs were differentiated into adipocte. 10days later, the mutant MSCs and the WT MSCs were red oil stained and QPCR test. But there's a...

13 June 2020 5,169 3 View

Does plastic waste as fiber reinforcement added into NWC will increase/decrease modulus of elasticity and compressive strength of concrete?

I was trying to gather any research which related to my question

10 June 2020 6,498 1 View

RNA extraction methods for virus w/o centrifuge?

20 April 2020 4,990 3 View

How to calculate drug compliance when topical administration?

Hi everyone, I am currently design an experiment about compare patients' drug compliance. The drug is used topically twice daily on the wound. We would provide diary card for subject to note the...

19 February 2020 6,891 1 View

Can I use one-way ANOVAs prior to multi-factorial ANOVA to reduce number of predictors?

My current experimental design involves several factors (e.g. Treatment, Enclosure, Night and Round), and involving all of them in a multi-factorial ANOVA would result in a large number of...

08 September 2019 6,913 2 View

Can preserved plant material still be processed for SEM analysis?

Hi all. I have root samples that are already preserved in 50% EtOH for 3 months. Can these still be further processed for SEM analysis? My samples are collected abroad and have to be in EtOH for...

03 September 2019 4,754 7 View

Proper imputation method for my missing data pattern?

Dear all, I am working with my resident survey data (n=8356) including 59 items, most of which are ordinal variables scored from 1 to 7, and others are continuous variables (e.g., age, residency...

24 July 2019 9,669 12 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View