I am trying to simulate phase change of refrigerant inside pipe extracting heat from panel in comsol. Can anyone help with how to simulate this?

My model is a rectangular multi layered structure under which refrigerant flows through cylindrical pipes. A heat flux of 600W/m2 applied on panel and internal forced convection is used for...

20 January 2021 9,478 1 View

Why does the ghost atoms gives error in NICS calculation with wb97xd in gaussian09 ?

In a trial, I am trying to calculate NICS (0 and 1) values for benzene ring using gaussian09. I have placed the ghost atoms at the center, 1A, and 2A above the rings. On using the method/basis...

19 January 2021 3,188 3 View

Rejected manuscript for journal publish after 1st revision, what needs to be done next?

Hello, My manuscript has been rejected by the editor and reviewers even after the 1st revision. Though I tend to disagree some of the points raised, assume I can't request for another revision...

28 December 2020 6,062 3 View

Acetylene reduction assay procedure?

Do anyone have the detailed step wise procedure(practical) to do the acetylene reduction assay? Or if anyone can let me know in which institute can i analyse my samples in India?

28 December 2020 6,717 2 View

What is the actual basis on which amount of Perovskites materials are to be taken?

In some discussion people took 112 mg of MAI and 461 mg of PbI2 in 1 mL DMF and some took 461mg of PbI2 too but 159mg of MAI there are many other authors took different amount of these materials...

17 September 2020 9,998 3 View

Forums to get online survey responses?

For my journal publish, I have been asked to increase the sample size by 200. I have been individually contacting more than 1000 Linkedin contacts and posted in all professional groups. But have...

04 September 2020 2,564 5 View

Derivation of Rice and Tracey equation???

12 July 2020 6,555 1 View

Can anyone suggest me where to get a low cost 2D Heterostructure Transfer System?

Except the 2D transfer system sold by HQ Graphene

09 July 2020 3,276 4 View

Why do mangroves intake oxygen through aerating roots like pneumatophores?

Many mangroves like Avicennia and Sonerratia species have developed pneumatophores as an adaptive strategy to survive in hypoxic environment. These pneumatophores receive oxygen from the...

16 April 2020 2,203 3 View

What is a popular book for understanding laser fundamentals and evolution and types of lasers?

I looking for books, channels, videos and PDFs regarding laser and particle interaction and evolution of laser power and laser types and how laser makes the physical changes on the ceramic...

05 April 2020 3,396 25 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Alternatives to traditional cloning of insert into backbone (dsDNA)?

Hi, I am stuck with a cloning experiment: The insert & backbone(bb) share one compatible end at 3' end(NotI) and one incompatible end at 5' end (XbaI for insert; HindIII for backbone). So i...

16 February 2021 1,664 3 View

Can part of recombinant protein not be expressed?

Hello. I am currently trying to express a recombinant gene with Avitag at the N-terminal. I'm aiming at a final product that looks like this: Avitag-(target protein) The protein I use is already...

14 February 2021 2,039 4 View

Transformation into the Stbl3 chemo competent cells?

03 February 2021 1,111 5 View

What is the best way and technique for serial dilution of bactria to get accurate number of colony ?

Hello I am infecting cells with bacteria in a well and then I am doing serial dilution of this well 1 in 10 and culture the two last dilution on MHA agar I have difficulty in getting accurate...

26 January 2021 9,256 5 View

Good day, I have not been able to amplify the plasmid Frt-hspB1. Somebody could give an advice me?

I acquired the plasmid in Addgene, reconstituted the filter paper where the plasmid comes from with 30 microliters of TE buffer pH 8, immediately afterwards perform the transformation of E.coli...

25 January 2021 2,526 3 View

Has someone used TTC agar overlay method and can help me?

I have tried to use TTC agar overlay method for a few times to identify respiratory-deficient yeast which were affected by EthBr. The problem is that cells which should have stayed white also have...

22 January 2021 585 10 View

I am having trouble with cloning my gene. How can I fix this?

16 January 2021 6,566 8 View

What is the best way to differentiate L. rhamnosus and L. casei in plate co-cultivation?

I'm having troubles in the differentiation and quantitation of each bacteria population during co-cultivation. The morphologic characteristics are very similar and it's really difficult to count...

13 January 2021 1,550 1 View