In in-vivo animal model, we calculate the dose in mg/kg body wt. How can we extrapolate the same in in-vitro evaluation? Is there any method or calculation to execute the same??
there is no real set formula or equation for conversion from in-vivo to in-vitro dose, check the literature for similar drugs/compounds in question.
This is tricky. The best way to do it would be to examine the blood concentration of the drug over a period of time (which should help you determine a half life as well). If it's a commercial drug, this information should be available with the product. Once this has been determined, you can perform a dose response assay on your cells in vitro. Using gene expression (for example), you can measure a gene that should be responsive to your drug, and compare that to results you've obtained in vivo. Probably not the answer you want (it's a lot of work!), but I hope it helps.
Scott
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