18 January 2022 4 9K Report

I am measuring chlorophyll with a handheld meter and want to convert the CCI readings to ug/mL to compare with an extraction method. Does anyone know how to do this?

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How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

How to constrain genetic algorithm input u?

My real system (buck converter) can only take an input of 0 to 1 (duty ratio) and I need to constrain the system so the controller action keeps within this bounds. How can I do this? The code for...

22 February 2021 2,125 3 View

The Role of Town Planners in CAV Use?

Colleagues and I are about to begin a project investigating the role of town planners and local authorities in establishing the introduction of connected and autonomous vehicles (CAVs). What...

21 February 2021 2,247 3 View

Why are the modelled and circuit system different in response for controlled buck converter?

[Images are attached at bottom] I am currently working on a buck converter controlled by two different control methods, Reinforcement learning applied to find PID values & a lead-lag...

16 February 2021 5,060 10 View

How can I interpret a ratio variable?

Dear colleagues, I am running a regression in which I have the following regression FoodExp = 67.04 DepRatio +.....e My interest is in the interpretation of the coefficient on the dependency...

16 February 2021 2,058 2 View

What is best technique to quantify necrotic cell death of specific cell populations?

I currently using FACS (Aurora Cytek) to quantify necrotic Gr1+ cell death from the spleens of immune competent mice. I target Gr1+ cells in the spleens of tumor-bearing mice using a photodynamic...

08 February 2021 733 6 View

Why do some full text requests not allow me to respond to the requesting person?

Over the last year, when I have clicked on 'View the Request' in the notifying e-mail for a full text request, I have been taken to the paper in question but there is no request visible and thus I...

16 January 2021 7,558 1 View

Spectrabase no longer has overlay function?

Hello, I am a quality control chemist and I test raw materials for a nutritional supplement company. I make heavy use of FTIR-ATR for identification. We are a small laboratory with a limited...

07 January 2021 8,850 2 View

Follow up tests to explore interaction effects in a multiple logistic regression: can someone help explain my contradictory results?

I am conducting a logistic regression in order to examine whether there is an interaction between the number of stressors participants were currently experiencing (between 0-9) and their current...

20 December 2020 1,043 9 View

How to stain adherent cells with DAPI?

Hello! I am growing transfected cells in 24 well plate. on the bottom of each well i have a small glass cover-slip. so the cells adhere to that cover slip. I am using this method because its very...

15 December 2020 7,121 3 View

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Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

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Can I use the Chlorophyll stability index (CSI) to calculate the stress potential of cyanobacteria that are under stress?

I want to calculate the stress potential of cyanobacterial cells that are inoculated with antagonistic bacteria. My results showed that there is a drastic reduction in the chlorophyll content of...

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Why and how to avoid negative values in optical density by spectrophotometry when measurements corrected by blank solvent?

I am trying to assess chlorophyll-a content from coral tissue using spectrophotometry (with SPECTROstar Nano, BMG). However, I always seem to obtain some negative values in my optical density raw...

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Why there is need to calculate the chlorophyll content and chlorophyll index. What protocol should I used for calculating chlorphyll in mustard?

I want to know the best and effective method/ protocol to calculate the chlorophyll from mustard plants. Please suggest some relevant and the methods or protocols from which I can easily calculate...

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Hello, can i learn amount of Chlorophyll in Spinach as a mg in 100 grams ?

I have looked on the internet and website but i could not find it so i need yours help please.

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What is it the relationship between photochemical efficiency and a chlorophyll a fluorescence in a plant ?

I hope somebody could help in explaining this point

10 December 2020 513 4 View

How can I use the selection matrix in Pymol to select atoms by their number within a cofactor?

I work with photochemical reaction centers (like Photosystems I & II) and it can be helpful to use PyMol to create useful pictures of them. With these proteins, the chlorophylls do all the...

04 December 2020 4,778 2 View

Simple clearing method for observation of hand-sectioned wheat leaves?

I am using a toluidine blue O preparation for staining different parts of wheat plants to observe a fungus growing in them. This works really well for lighter tissues of the plant! However, the...

27 October 2020 9,159 1 View

Whether it is compulsory to carry out in-situ water sampling and laboratory-based analysis for a water quality study based on remote sensing?

A study assessed the water quality status using a few parameters such as Chlorophyll a, BOD, COD, TSS and etc to determine the pollution level of water bodies using remote sensing techniques....

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Is it possible to carryout Chlorophyllin (Mg-Chl, or Cu-Chl) photosensitized degradation of methylene blue dye in presence of visible light ?

As both Chlorophyll semi-synthetic derivative and methylene blue organic dye has been widely used as a potential photosensitizer in applications like PDT but I want to know that; a chlorophyll...

15 August 2020 6,325 3 View

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