I have measured the lifetimes of my compound at some emission wavelengths using single-photon counting technique and emission spectra. Is there any free fluorescence software or computer program that can construct time-resolved emission spectra?
1. Collect all lifetime decays (at different wavelength) for constant time period say 200 sec.
2. Get the ASCII file. X - axis is time (ns or may be ps) and Y - axis is your intensity.
3. Now you have intensities (at different wavelength) corresponding to different time. Now you have fluorescence emission at different time e.g: 0 ns, 0.1 ns 4 ns. Plot these spectra at different time and get your desired TRES.
Other way: Our paper "Estimation of kinetic parameters from time-resolved fluorescence data: A compressed sensing approach " (http://pubs.rsc.org/en/content/articlelanding/2012/RA/c2ra21773b#!divAbstract) give a very fast and usable method! :) If you have a questions, please contact with G. I. Groma.