Can I use Potassium clavulanate " VETRANAL™, analytical standard (Sigma-Aldrich) Code 33454 to confirm ESBL E.coli. ?
Dear Apirom,
For sure you can use it.
For the use of clavulanic acid (CA) as ESBL inhibitor, please see see the following publication:
J Clin Microbiol. 2009 Nov; 47(11): 3409–3412.
Published online 2009 Aug 26. doi: 10.1128/JCM.01141-09
PMCID: PMC2772648
Broth Microdilution Method To Detect Extended-Spectrum β-Lactamases and AmpC β-Lactamases in Enterobacteriaceae Isolates by Use of Clavulanic Acid and Boronic Acid as Inhibitors ▿
This study was designed to evaluate the performance of the broth microdilution (BMD) method to detect production of extended-spectrum β-lactamases (ESBLs) and AmpC β-lactamases in Enterobacteriaceae by using clavulanic acid (CA) and boronic acid (BA) as ESBL and AmpC β-lactamase inhibitors, respectively. A total of 100 clinical isolates of Enterobacteriaceae were analyzed. Mueller-Hinton broth containing serial twofold dilutions of cefotaxime (CTX), ceftazidime (CAZ), aztreonam (ATM), or cefepime (FEP) with or without either or both CA and BA was prepared. An eightfold or greater decrease in the MIC of CTX, CAZ, ATM, or FEP in the presence of CA and BA was considered a positive result for ESBL and plasmid-mediated AmpC β-lactamase (PABL), respectively. In tests with CA, expanded-spectrum β-lactams containing BA (CTX-BA, CAZ-BA, ATM-BA, and FEP-BA) showed higher positive rates in detecting ESBL producers than those without BA. The combination of CTX- and CAZ-based BMD tests with CA and BA showed sensitivity and specificity of 100% for the detection of ESBLs and PABLs. The BMD testing could be applicable for routine use in commercially available semiautomated systems for the detection of ESBLs and PABLs in Enterobacteriaceae.
To view the full publication, please use the following link:
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2772648/
Hoping this will be helpful,
Rafik
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