15 August 2024 1 9K Report

I have 4 files from different genes (ITS, TEF1, TUB2, RPB2) and need to concatenate aligned sequences from around 35 strains in order to make a pnylogenetic tree. Is the a software available for this or any other way?

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30 August 2021 7,680 3 View

When is the right time to stop training a classification algorithm?

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27 January 2021 6,010 3 View

Designing Taqman primers, probes - differentiating species - what's more important?

I need to design Taqman probes for different species of the same genus in order to differentiate between them. I have read the guidelines (no 5' G end on probe, max 2 GC 3' end primers, ~10C...

29 December 2020 5,044 1 View

Preparing and reviving mycelial/ conidial glycerol stocks - how to and tips?

I have an issue with reviving mycelial/ conidial glycerol stocks from the -80 freezer. The fungi I'm working with belong to the Botryosphaeria and Neofusicoccum species. I prepare the conidial...

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Category 3 PRIIP MRM calculation - How to derive risk-neutral returns from the bootstrapped historical simulation returns?

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