Stavros Palavouzis

5 Questions 1 Answers 0 Followers

Questions related from Stavros Palavouzis

Is it possible to blast sequences with blast discontinous sequences with ncbi blastn?

I might be going the wrong way about this, but is there a way to check if two or more sequences are present on the same gene? The reason I want to do it: I've designed primers and taqman probe...

07 November 2021 7,157 2 View

While submitting ITS sequence on NCBI how do I include CDS data?

I have to submit ITS sequences (but the principle should be the same for every gene) on ncbi. How do I include CDS data. For ITS it is: 18s, ITS1, 5.8s, 28s? I tried using the default tool but it...

31 August 2021 7,665 3 View

Designing Taqman primers, probes - differentiating species - what's more important?

I need to design Taqman probes for different species of the same genus in order to differentiate between them. I have read the guidelines (no 5' G end on probe, max 2 GC 3' end primers, ~10C...

30 December 2020 4,998 1 View

Preparing and reviving mycelial/ conidial glycerol stocks - how to and tips?

I have an issue with reviving mycelial/ conidial glycerol stocks from the -80 freezer. The fungi I'm working with belong to the Botryosphaeria and Neofusicoccum species. I prepare the conidial...

06 December 2019 9,352 0 View

Why did I get multiple bands in my southern blot?

I used 15 genomic DNA samples and a 1064 bp probe to screen for mating types. The enzyme I used to cut the DNA was HindIII which did not cut (according to Sequencher) the probe. Also, I have...

31 October 2015 7,586 3 View