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Questions related from Stavros Palavouzis
I need to convert multifurcating phylogenetic trees generated from MrBayes to binary trees so that I can use them in Phastweb. Anyone knows a straightforward way (online if possible) to do it?
30 November 2024 852 0 View
I have 4 files from different genes (ITS, TEF1, TUB2, RPB2) and need to concatenate aligned sequences from around 35 strains in order to make a pnylogenetic tree. Is the a software available for...
15 August 2024 9,347 1 View
I might be going the wrong way about this, but is there a way to check if two or more sequences are present on the same gene? The reason I want to do it: I've designed primers and taqman probe...
07 November 2021 7,230 2 View
I have to submit ITS sequences (but the principle should be the same for every gene) on ncbi. How do I include CDS data. For ITS it is: 18s, ITS1, 5.8s, 28s? I tried using the default tool but it...
31 August 2021 7,732 3 View
I need to design Taqman probes for different species of the same genus in order to differentiate between them. I have read the guidelines (no 5' G end on probe, max 2 GC 3' end primers, ~10C...
30 December 2020 5,084 1 View
I have an issue with reviving mycelial/ conidial glycerol stocks from the -80 freezer. The fungi I'm working with belong to the Botryosphaeria and Neofusicoccum species. I prepare the conidial...
06 December 2019 9,432 0 View
I used 15 genomic DNA samples and a 1064 bp probe to screen for mating types. The enzyme I used to cut the DNA was HindIII which did not cut (according to Sequencher) the probe. Also, I have...
31 October 2015 7,628 3 View
