How can comparison between phosphorylated and total protein amounts be made without stripping and re-probing the same gel but using two gels, one for phosphorylated and the other for total protein? Both gels have the same loading controls.
you would need a densitometer to measure the expression of the 2 different WB
Do you have a standard curve ? Then you can quantify them Exp Anim. 2011;60(2):193-6.
Use of sample mixtures for standard curve creation in quantitative western blots.
Suzuki O, Koura M, Noguchi Y, Uchio-Yamada K, Matsuda J.
Source
Laboratory of Animal Models for Human Diseases, National Institute of Biomedical Innovation, 7-6-8 Saito-Asagi, Ibaraki, Osaka 567-0085, Japan.
Try this site
http://www.dwalab.ca/labman/misc_methods/WesternBlotting.html#QuantitativeWesternBlotting
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