After pulp extirpation was done and the bleeding from the canal controlled, sterile paper points were introduced into root canals and were transferred to Staurt’s transport medium. 2 ml of whole unstimulated saliva was collected from each patient into sterile plastic containers by the “spitting method.” In the case of symptomatic endodontically treated teeth, the root canal filling was removed, and sterile paper points were introduced into the canal and transferred to the transport medium. The saliva and the root canal culture samples were inoculated within 4 h onto Sabouraud’s agar plates and were incubated for 48 h under anaerobic conditions. The totals colony forming units (CFU) were counted using the colony counting machine. Obtained colonies were stained with periodic acid-Schiff stain to examine the organisms. “Germ tube test” was done to confirm that the fungal organisms were indeed C. albicans.