How do i analyze scratch assay data obtained thru imageJ?

hello. i manually measured the area in each image. the output i got was area/mean/min/max then i put all the data into excel, and also added the following columns: group names/date/hour pic was...

19 January 2021 3,936 3 View

What are the alternative to Rotary Evaporation?

I want to remove solvent to obtain a solid . i would like to remove solvents such as THF, TEA, carbon tetrachloride . the obtained solid which is a Phosphorus ester. What could be an suitable...

04 January 2021 1,220 1 View

ENVI error when opening Metadata?

ENVI software (V 5.3) has a problem with opening some Metadata files. An error appears which means it does not seem to be a Metadata file. How can I solve this problem?

20 December 2020 4,590 3 View

How to detect saturated pixels in Landsat images?

I have heard pixels with DN>10000 in Sentinel-2 images are saturated pixels. How to detect saturated pixels by DN in Landsat images? Is there any reference that has determined these DN numbers for...

16 December 2020 5,827 3 View

What is the role of Phytomelanin in seed coat?

We know commonly found in seed coat at Asparagales and Asteraceae family But, what is the role of Phytomelanin in the seed coat? Is it only a protector?

03 December 2020 7,493 5 View

Do NPC or FC structures have power loss of capacitor ripple?

I should say that when capacitors are parallel with input voltage DC, they have capacitor ripple voltage loss cause of direct connection but when a capacitor is in path of charging current -it's...

04 September 2020 9,774 1 View

In gas chromatograpy, how do I propagate SD of 5 readings at each standard conc. along with the SE of the coefficients in a quadratic regression?

Hi, This question pertains to gas chromatography to measure levels of carbon monoxide using reduction gas detector technology. I have developed a quadratic regression using 6 standard CO...

11 April 2019 7,888 3 View

I have a check box question, where the respondents can choose more than one choice, how can I insert more than one choice in the SPSS?

I have a check box question, where the respondents can choose more than one choice, how can I insert more than one choice in the SPSS extraction sheet? e.g: 1. medical 2. surgical 3....

27 February 2019 4,397 3 View

Nuclear extraction from brain tissue?

Hi, I'm looking for an inexpensive and efficient method to isolate nuclear fraction from brain tissue. There are kits and protocols available, but they are mostly for cells and tissues other than...

14 January 2019 402 2 View

What are the basic characteristics of Big Data?

I collected more than 200000 samples, but someone said the data was not a Big data. I asked him to define the Big data, unfortunately he could not define.

15 August 2018 4,985 12 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View