22 September 2017 3 5K Report

I'd like to fuse 3 fragments into a vector, but they have RE sites. How to connect them together without adding extra space? I don't wanna additional a.a to be translated.

More Suipwksiow Wjkp's questions See All
How to validate anti-human-immune-checkpoint in mouse model (in vivo)?

The antibody is against human protein, but with mouse IgG (as it is generated in mouse). Before totally humanizing it (which takes a lot of money and time), I want to validate its function in...

20 April 2022 9,928 0 View

Can FDA-approved drugs be used in other disease setting?

Hi Everyone, I'm exploring the anti-tumor effect of some drugs. I'd like to test some FDA-approved drugs which are not used to treat cancer. My question is, if there is any anti-tumor effect for...

14 December 2020 1,630 1 View

How to do proper human T cell proliferation assay?

Hi Everyone, I did numerous human T cell CFSE proliferation assays, but the peaks never shift. I put CD3/CD28 and IL2, and I can see that T cells did proliferate by my eyes (as there was increase...

08 December 2020 9,238 3 View

How to know if my protein forms a dimer or not?

Hi everyone, The protein I studied is a membrane protein. Some study indicates that there is possibility that this protein can form a dimer. I did standard western blot for this protein and my...

20 October 2020 7,150 7 View

What assay should I do to confirm membrane protein-protein binding?

Hi all, My hypothesis is that CD99 binds to CD88 (two membrane proteins). My colleagues suggested me to do co-IP, but I don't have good western blot antibody against one of the protein. However,...

14 October 2020 9,377 6 View

How to ensure a marker antibody won't activate T cells and affect the result?

I'm recently buying some anti-CD3 and anti-CD28 to activate T cells. I also notice some of this clones have been conjugated with fluorescence for the purpose of labeling T cells for flow...

06 October 2019 7,384 3 View

To synthesize chimeric receptor, should I used real gene sequence or artificial sequence?

I'm synthesizing a chimeric antigen receptor (CAR), and I have the whole sequence of the amino acid. Now the problem is, to ask company to synthesize the DNA of the CAR, should I send real gene...

06 February 2019 1,641 1 View

Why my gene in single-cell sequencing showed zero value?

I have many single-cell sequencing data. I checked my gene of interest in these data. However, all of these single-cell sequencing data showed that my gene has no expression, no matter in immune...

03 January 2019 1,876 3 View

Why can't I detect the surface protein by western blot?

I was doing an over-expression experiment of a gene for a year. It is a surface protein. The biggest trouble is that I can never detect the gene overexpression by western blot. Interestingly, when...

29 November 2018 6,457 3 View

For HDAC inhibitors, which HDAC member(1/2/3/4...) is inhibit by it?

There are so many HDAC family member (1-11), but which is the one that is inhibited by HDAC inhibitor?? I ask this question because I found some of them seemed to be tumor suppressor, while some...

25 March 2018 652 2 View

Similar questions and discussions
Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

Is there an English Translation of the Carl Moller text: ZUR VERGLEICHENDEN ANATOMIE DER SILURIDEN?

I recently came across an anatomy text by Carl Moller that was published in 1915 but it is in German or Dutch neither of which I can understand. I would like to know if there is an English...

10 August 2024 4,347 1 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How can major oxides determined by ICP-MS/ES be normalized to 100% volatile free? How does this connect with LOI?

I am analyzing cherts from different sources found within one specific geologic formation using ICP-MS/ES. My committee was asking this question about my analysis. I was not sure how to answer this

05 August 2024 7,902 3 View

Is it true that the vacuum bomb is about to arrive?

We assume this to be true. We also assume that the vacuum bomb is the latest version of explosives with an explosive power of a few to ten kg of TNT equivalent. It has the unique characteristic of...

04 August 2024 4,534 1 View

How to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field in Techplot?

Dear Researchers I need to know, how to load and plot 2D-PIV (particle image velocimetry) recorded velocity vector field data in Tecplot? Thank You

02 August 2024 3,615 1 View

How can we now if the promotor in the vector is well?

Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...

02 August 2024 3,987 1 View

How do environmental toxins interact with genetic predispositions to increase cancer risk?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 2,627 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close