I eluted the polar compounds from the stem bark extract with methanol but it left a brown stain on the sephadex LH-20, how do I clean the stain? Thank you!
I'm really curious with how Maxent setting work. I'm using it for elephant habitat mapping. I'm using various variable but I got a problem there. So the first picture is my output, and the second...
21 January 2024 3,953 5 View
Actually this is how i do. 150mg (pure extract) dilute with 3ml=3microlit (DMSO) will get 50mg/ml(stock solution). How can i calculate the concentration?
25 June 2023 6,599 1 View
Actually i used plant extract to determine whether there is antibacterial activity or not.
14 May 2023 5,204 4 View
i just knowing the equality of Habitat preference index from a book HPI = Px/Pa, Px is the percentage of the population that is in the x habitat & Pa is the percentage of the x habitat area...
13 December 2022 4,292 2 View
I am using a mammalian cell line (Vero) and need to infect the cells with a virus resulting in reduced cell number at the end of the experiment. I plan to standardise either the number of cells...
06 December 2022 3,581 2 View
Hello, everyone, i have some question about MORISITA INDEX does 'n' mean the number of plots per village of one sample area that I use? if it's true, what about a sample of village of an area? it...
17 November 2022 3,439 0 View
I want to include AA/Vit. C as a positive control for my antioxidant study using Vero cells to compare with my drug compounds. I will measure MDA (lipid peroxidation marker), SOD, and Catalase...
26 July 2022 6,045 2 View
I will need to harvest different batches of cell samples at different time points. Then I will perform homogenization on a separate day to get the cell lysate and run the assay using all samples...
02 February 2022 2,207 0 View
I'm currently coping with qsar analysis of amino-alcohol derivatives of xanthone. In accordance with the best QSAR equation, the position of the substituents were replaced and chlor substituent...
18 February 2021 9,288 3 View
I am currently evaluating my ecobrick activity. I created this Ecobrick by compressing plastic packaging waste into a bottle to a certain weight, for that, I want to calculate how much carbon I...
09 March 2020 10,096 4 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
Hi everyone I need a file with a dirty and clean potato image
04 August 2024 7,199 4 View
If you think of electrons with spin as bar magnets, you know bar magnets of opposite polarity as long as they're not occupying the same spatial location don't cancel out each other's magnetic...
03 August 2024 3,927 2 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
The module of the CAD detector is corona Veo. It has been idle for a long time. And it was not well maintained before idle. I have replaced the gas filters and flushed the system with a 50/50...
01 August 2024 7,805 0 View
Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...
29 July 2024 8,624 1 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View
We are working on a robot that cleans solar panels using fresh water supply and a rotating brush. We are trying to conserve as much water at possible by recycling the dirty water that is collected...
28 July 2024 5,778 2 View