Hi everyone! I have a problem with the seletion of the primers for a Multiplex PCR. If I have a large amount of data of fragments size amplified by diffrent primers, how can I choose the most easy way the best primer combinations?
Prasanta Purohit
SC Amold et al (2001) a rapid and reliable 7-deletion multiplex PCR assay for alpha- Thalassemia. Blood, 98(1): 250-251.
please follow this article this will help you.
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Prasanta Purohit
SC Amold et al (2001) a rapid and reliable 7-deletion multiplex PCR assay for alpha- Thalassemia. Blood, 98(1): 250-251.
please follow this article this will help you.
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Areli Munive Olarte
you can use software like clustalx, gene doc , mFold, primer 3, and finally blast
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