I am trying to quantify a highly transcribed RNA to normalize transcription and I thought about ribosomal RNAs (rRNAs).
I thought that 18S-28S rRNA should work, but what ID exactly?
Candidates: 18S ribosomal RNA AT2G01010
versus: rrn18 Gene description 18S ribosomal RNA (I think the last one is mitochondrial, am I right?).
Would it make sense to measure also 25S rRNA? They apparently are transcribed in tandem.
¿Another highly expressed RNA to consider as a possibility?
References welcomed!
Thank you!