This is about phytoremediation of mercury contaminated site. In this regard, i am going to check the bioavailable fraction, which include water soluble fraction and exchangeable fraction, which will tell the possibility of mercury to be accumulated by the plants.
Thanks due to your questions. Only few geo chemical species of metals (for example water soluble, exchangeable, carbonate bound, Fe-Mn bound and organic material bound) are labile/bio-available i.e., fraction of metals that can be available to the organisms.
As you are interested to investigate the concentration of Hg in the water soluble fractions and exchangeable fractions ., the possible methods are as follows;
1. Water soluble fraction can be separated using water or hot water following Svete et al. (2000);
2. Exchangable fraction (using 1 M ammonium acetate or 0.01–0.5 M calcium chloride; pH 7 ) following Tessier et al. (1979).
>>Svete, P., Milacic, R., & Pihlar, B. (2000).Annali di Chimica, 90(5–6), 323–334 Rome.
>>Tessier, A., Campbell, P. G. C., & Bisson, M. (1979).Analytical Chemistry, 51(7), 844–851
Please check the possibility of contamination by small organic species such as methylHg
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