You can measure the congo red dye concentration using UV-Vis at λmax = 498
You have to dissolve the adsorbent into solvent (eg water), then take the UV spectrum. You might need to adjust pH, since the dyes seems pH sensitive!
https://en.wikipedia.org/wiki/Congo_red
Article Plausible Applicability of Aqueous Congo Red Dye System as S...
E-Journal of Chemistry Volume 5, Issue 3, Pages 572-576 http://dx.doi.org/10.1155/2008/809362
Plausible Applicability of Aqueous Congo Red Dye System as Secondary Gamma-ray Dosimeter
I. Das Sarma and D. V. Parwate
Department of Chemistry, Rashtrasant Tukadoji Maharaj Nagpur University, Nagpur – 440 033, India
Received 18 November 2007; Accepted 10 January 2008
" Aqueous congo red dye solutions in the concentration range of 0.01–0.03mM were irradiated for varying γ doses 0.050 kGy to 0.150 kGy. The molar extinction coefficient of the dye solution of congo red was found to be 2.70×105 at its absorption maxima 499.0 nm. "
Hany Fathy Heiba thank you i also want to know about calculations
how to calculate the adsorption
1. Record the VIS spectra for Congo Red and select the maximum wave length.
2. Prepare a calibration plot (with at last 4 etalon solution with known concentration of Congo Red).
3. Measure the absorbance for you solutions at maximum wave length, and from regression equation of it can be determined the Congo Red concentration at equilibrium.
4. Calculate the adsorption capacity.
Actually there are two ways to calculate the adsorption — 1- Removal % = (Intial concentration - Concentration at any time)/intial concentration * 100 .... 2- Adsorption Capacity= late the adsorption — 1- Removal % = (Intial concentration - Concentration at any time)*Solution volume(L)/ adsorbent mass (g)
Please check my profile and my publications for more information
Nelson Pynadathu Rumjit
Laura Bulgariu98% removal means that you have removed 49 ppm from your initial 50 ppm and the remaining conc is 1 ppm and the capacity = (Ci-Ct)*V(l)/m(g) so
Removal capacity = (50-1)*0.05/0.06= 40.83 mg/g
- Badges
- Science topic
- Similar topics
- Chemistry
- Biochemistry
- Biochemical Techniques
- Blotting Techniques
- Immunoblotting
- Dyes