we are working on agrobacterium transformation,bacterial culture got small clamps, we want check purity of culture....
I would streak it out on a plate+ATBs and start over from a single colony...
1. Are you growing LBA4404 in LB medium? I have experienced these 'clamps' when I grew LBA4404 in pre-mixed commercial LB medium. The situation was improved when I cut down the NaCl (one of the 3 components in LB) amount and prepare my own LB.
2. To check Agrobacterium, you can also take a look at this discussion on RG recently: https://www.researchgate.net/post/How_can_I_identify_Agrobacterium_tumefaciens_LBA4404_strain_via_colony_appearance
If your batch culture is clumpy try 523 media instead of LB. The magnesium in 523 helps prevent clumps in certain strains.
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