I am having a problem in studying the degradation since while we record UV-Vis spectra of solution. Absorption of nanoparticles along with dye comes in the spectrum. To study the degradation I have to record only the Dye.
Please suggest the methodology?
Thanks in advance.
Dear Shailendra Sharma,
What you should do is the following:
1 mL of the suspension (nanoparticles with the dye) is withdrawn periodically and centrifuged to remove the undissolved material. UV–visible spectra of the dye samples are recorded with UV–visible spectrophotometer (the resultant solution should be clear for adequate UV measurement). If after centrifuge, the solution still unclear, you should filter through Wattman filter paper.
For more on similar procedures, you can be assisted with the paper contained in the following link (see methods section):
https://www.researchgate.net/publication/260715418_Enhanced_photocatalytic_degradation_of_methylene_blue_using_biologically_synthesized_protein-capped_ZnO_nanoparticles
Hoping this will be helpful,
Rafik
Article Enhanced photocatalytic degradation of methylene blue using ...
You follow Prof. Rafik,s method and also if you will get the peaks, you can do GC-MS/HPLC to quantify and know your things.
First of all, you want to separate the nanoparticles from the dispersion using by means of centrifugation/syringe filter etc. Using the filtrate, you can measure the absorbance using a UV-vis spectrometer. For mechanism or reaction pathway studies, it's better to use chromatography method for analysis.
Dear Shailendra
I will suggest you the best method in my Idea. I think photocatalytic dye degradation experiment can be carried out better by using your nanoparticles as a thin film. As a matter of fact at the first its best you make your nanoparticles paste to fabricate a porous thin film using dr blade method. After this stage its time to immerge your thin film in a pottery dish of your dye solution. now you should put your dish in a dim place. Then take a UV-Vis spectrum and then put dish under the lamp for 10 min andagain take a new spectrum. repeat this until spectra would have smoothed.
I recommend this good article. im sshure it will help you.
https://www.researchgate.net/publication/265390767_High_Efficiency_Photocatalytic_Mineralization_of_CI_Reactive_Red_2_using_Transparent_Self-Assembled_Nanopin-Like_Titania_with_High_Preferential_101_Orientation_on_Alumina_Thin_Film_in_Aqueous_Environm
Article High Efficiency Photocatalytic Mineralization of C.I. Reacti...
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