I am sorry but your question seems to be incomplete.
Are you looking for the experimental procedure for TP/TF?
Or you have the procedure and need calculation for it in terms of Wt/Wt?
If you only need procedure, you can get it in any publish article. If your talking about calculation then you have to provide calibration curve for the assay and corresponding value of the sample extract you are using.
I have done flavonoid and phenolic content of plant extracts. Might that formula u can used for fungal extract also. I am sending my publication that might help. Also take maximum precaution because it's fungus can get contaminated easily by other atmospheric flora.
Please tell me what standard/ u r taking for comparing...
For calculation you may use graphpad calculator to do t-test, anova etc.
Hope this will help you in your research work!!
http://www.graphpad.com/quickcalcs/ttest1.cfm
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