How to calculate total carbohydrate content (anthrone method) from the standard curve ?

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Simon Miguel Lopez

Hello Anshuman Nath

I think whether you include C=0 or not, your final regression equation would look like Y=mX. So you can proceed with this equation to calculate for your total carbohydrate content of your sample.

Hope this helps.

Best

Kavnen Tseke

Hi Anshuman Nath

I plot my standard curves in Microsoft excel and it generates the y=mx+c equation for the curve automatically with the appropriate value for c. I then use the standard curve equation to solve for the unknown concentration. Hope this helps.